Transcription-Factor Library: Coming Soon in 2011 

In 2009 we proposed to NIH we would generate an homogenous gold standard GATEWAY^TM compatible collection containing every Arabidopsis TF. The corresponding coding sequences will be cloned in the same vector using the available ORFeomes as the template resource when possible. The remaining 25% missing ones will be generated by following different complementary amplification protocols and subsequently cloned in the same vector backbone. In addition, we propose to create and distribute to the community, nine application-ready genomic collections containing each TF in fusion with different tags for a multitude of applications. These nine collections will allow 1) overexpression screens in planta of wild type, and EAR or VP64 translational fusions  2) Protein-protein interaction screens 3) Protein-DNA interaction screens 4) Subcellular localization and 5) bacterial recombinant protein expression.

For further information, please contact Steve Kay  skay@ucsd.edu

See the Agilent Integrated Systems robot (BioCell 1200) in action on You Tube

Applications

1. High-throughput screening

2. Compound management

3. Genomics: DNA extraction, PCR sample preparation…

4. Cell-based assays

5. Microplate-based cell maintenance

6. Secondary screening and ADMET assays

7. Enzyme assays and more…