-- PROGRESSIVE (similarity) ALIGNMENT -- Blosum62 Sequences are entered in the following order: 1)GRA2 GRA2BSU.TXT 2)ORFC ORFCBSU.TXT 3)ORFD ORFDBSU.TXT 4)GRB2 GRB2BSU.TXT 5)YNDE YNDEBHA.TXT 6)GBME GERABBME.TXT 7)GRKB GRKBBSU.TXT 8)GERI GERIBBSU.TXT 9)GERX GERXBBAN.TXT 10)YFKT YFKTBSU.TXT ---- Alignment ---- *** (gap penalty = 6) *** GRA2 M S Q K Q T P L K L N T F Q G I S I V A N T M L G A G L L T L P R A ORFC M S Q K Q T P L K L N T F Q G I S I V A N T M L G A G L L T L P R A ORFD M F S P T S K I T T A Q A T I I I I N Y M L A A G V L T L P R T GRB2 M R K S E H K L T F M Q T L I M I S S T L I G A G V L T L P R S YNDE L N R N N N Y T I D P L E I S I A L I A F I I G V G I L T L P G S GBME M E K A K I S A T Q L F I L M V L F E L G S A L L V P L A GRKB M E K A R I S I R Q L F V M I I I F E L G S S L L I T P G S GERI M S K V Q E K Y Q I S P I F V F F L I H G A Q F G A G V L G F A R YFKT M D K T S A Y Q G L F F G A L Y T L A V G L K H A P I L GERX M A K Q M V N F F Q I A L V L I G S T G I I N H V I I I P M L GRA2 L T T K A N T P D G W_I T L I L E G F I F I F F I Y L N T L I Q K ORFC L T T K A N T D G W_I T L I L E G F I F I F F I Y L N T L I Q K ORFD V T E Q T Q S P D G W_I S V L L G G V L A V I A G M I I A K L GRB2 A A E T G S P S G W_L M I L L Q G V I F I I I V L L F L P F L Q K N YNDE L A D T L N T A D G W_I T I I V G G S I I C A L V Y V T V R L Q K GBME M G A K Q D A W_L A I L I G M L C S F F F F L I Y H K L GRKB M A G R D A W_I A V L L G C A I G L F L F Y L Y Q G I GERI I T A K A A G Y D G W_I G V V I T G I V I H V L M W M M Y F L L R E T YFKT M I E S A K Q N A W_H S Y I L G V V I V I P A L W L M H R L M K K GERX L D H S G R D S W_I S I I I L S L V Y I I W I P C V F I V H K Y GRA2 K H Q Y P S L F E Y L K E G L G K W I G S I I G L L I C G Y F L ORFC K H Q Y P S L F E Y L K E G L G K W I G S I I G L L I C G Y F L ORFD S Q Q Y P K E T F Y E Y S R H I V G K W L G H L I S I V F I T Y F L GRB2 S G K T L F K L N S I V A G K F I G F L L N L Y I C L Y F I YNDE E F P E Q T L L Q F L L A G Q K S K W L A V L L G V L F S L H F L GBME Y S Y Y P D L L P T E Y M Q K I L G K V L G T L L A F I Y I V Y F L GRKB Y Q C Y P N S S P K E Y M D D M L G T K L S W L F S F L Y I L Y F A GERI N G N L I D L Q R Q T F G K W I G N V F N I I F A T Y F L YFKT H Q D K N I Y E L L S D S S P I A G R I I I L L F S L Y F L GERX T R E E H L F S W L M R N Y G G F I T Y P L L S I I V L Y L I GRA2 G V A S F E T R A M A E M V K F F L L E R T P I Q V I I L T F I C C G ORFC G V A S F E T R A M A E M V K F F L L E R T P I Q V I I L T F I C C G ORFD A L G A F E V R V M S E I V D F F L L E G T P S W A I I M T V L W I G GRB2 G I V C F Q A R I L G E V V G F F L L K N T R M A V V V F I F L A V A YNDE M L M G F E S R M L A I V L D M Y L I D R T P S V I V I C T I L V V T GBME Y D A S R V L R D F G E M L L T F A Y P D T P L F I A N A L L M L V I GRKB Y I A A R V L R D F G E M L L T F A Y H D T P I I I V N A L L M V V S GERI I V S I S V I R T Y V E I I Q V W M F P T A S T F M L T L F L C L V S YFKT L I N A H D I R F F I N L I N I L F L P R T P M A V L G G V I I F V A GERX I L G T V T L K E T L T F F S F Y L P E T P R I L L G V L L S I I C GRA2 I Y L M V G G_L S D V S R L F P F Y L T V T I I I L L I V F G I ORFC I Y L M V G G_L S D V S R L F P F Y L T V T I I I L L I V F G I ORFD L Y S I T Q G_L D P I A R L F E M I F P I T V I I F L T I A L M GRB2 I Y H V G G G_V Y S I A K V Y A Y I F P I T L I I F M M L L M F YNDE S Y A V S K G_V Q G L I H L N V L F I P F I L T V L L A I L L F GBME I Y I T R K G_I E A I A R S G E L L F I F M Y F L A I A G F I L I I GRKB I Y A V R K G_I E V L A R A A E L L F G A M Y L L G A I G L V L I I GERI Y Y I I S S G_F R V I T G I C V I S V G G T L G Y L F L S L F YFKT I C I A R E G_K E T L T R M A Q I F L F P F G I L V L F L P F T GERX F Y N I Q R G_V Q S I A L T T G I L L P V V F L L G F F V M I A GRA2 S F K I F D I N N L R P_V L G E G L G P I A N S L T V V S I S F L G M ORFC S F K I F D I N N L R P_V L G E G L G P I A N S L T V V S I S F L G M ORFD S L G I F E I N N L R P_V L G D G I M P V L R G V K T T N L S F T C S GRB2 S F R L F Q L D F I R P_V F E G G Y Q S F F S L F P K T L L Y F S G F YNDE N V Q H A K L D P I L P_V A P K G I L H P L K G L P N V V L A Y I G V GBME S S G L I D F K N L Q P_V L K E G I L P V A K V A L T E T L Y F P F T GRKB V S G T I D P H N L K P_V L A N G I S P V L H S V F T Q T M Y V P F G GERI V L K Y S H W E N L L P_I F T H S F S D I L K S A Q L S I Y S M T G F YFKT L A T Q I E L Q N L T P_V F E G L I P Y L Q S G Y Y A F G T M G GERX N F P H K D Y S L L K P_I M E H G M D P V I K G M I Y P A A G F V GRA2 E_V M L F L P E H M K K K K Y T F R Y A S L G F L I P I I L Y I L ORFC E_V M L F L P E H M K K K K Y T F R Y A S L G F L I P I I L L F L ORFD E_I M F I L V A F M K K P K N A V K A V V I G T G V V T S F Y M I GRB2 E_I I F Y L V P F M R D P K Q V K K A V A L G I A T S T L F Y S I YNDE E_I L L F F M G R M K G K D V K A W P L N I G V G I V T L F Y F L GBME E_A I V F T M L L P Y L S N P K K A N V T M L C A T G L S G I N L I I GRKB E_V V L F V M I F P N L N D R K D V K K M G M I A M A I S G L I V A L GERI E_I Y L M V Y P F V K N P K Q S H K F A Q Y G A L F S N L L Y L F YFKT E_L I I L P L L F S N R S V P L K Y T I F A I L L G A L L L A V GERX E_L I F I L F L Q H H I G S K I K L S Q L I I V G I I L A G I T L G P GRA2 T Y I I V V G A L T A P E V K T L I W P T I S L F Q S F E L K G I F I ORFC T Y I I V V G A L T A P E V K T L I W P T I S L F Q S F E L K G I F I ORFD T M I M V I G A L S V E G V V T R T W P G L D L M R S F E I P G L I F GRB2 T L L I V I G C M T V A E A K T V T W P T I S L I H A L E V P G I F I YNDE I T F F T Y F V F G V E Q S K V I N F T T V E L A K V V E V P G G F F GBME T M I I N I S V L G V D L T T R S Q F P L L S T V Q T I Q V A D F L GRKB T V A I N I S V L D V D L T L R S Q F P L L S T I Q T I K V E E F L GERI S T L L A F S F F S E K Q L L K T I W A Q L S M T Q V V Q L P F I YFKT M L F S S I S V F G P N L T S T F F D P A Y M V I R Q I R I T D F L GERX T I A A I V E F G P F V A A N Q R Y P T F E E W R L V S I G K Y I GRA2 E R F E S F L L V V W I I Q F F T T F V I Y G Y F A A N G L K K ORFC E R F E S F L L V V W I I Q F F T T F V I Y G Y F A A N G L K K ORFD E R F E S F L L V I W I M Q L F A T F I I T F Y A A S L G V S Q GRB2 E R F D L F L Q L T W T A Q Q F A C M L G S F K G A H I G L T E YNDE E R V E S M M I T V W T M A I F N T I A M S Q L I I I L I Y N D L F S GBME E R L D V F F M F A M V I G I Y F K V S I L F Y A S I I S T A T GRKB D R L D V F F M L A L I I G G F F K V S L Y L Y A T V V G T S T GERI E R L E Y I A I S A Y A L V I I T S F I L P L W A A T R A T H E YFKT D R S D L I I A A F W I P V I M V K I A G S L Y I V V Y G L S F GERX E H L D F L S V Y Q W L V G V F I R I S L V I F L I P D V L Q GRA2 T F G L S T K T S M V I I G I T V F Y F S L W P D D A N Q V M ORFC T F G L S T K T S M V I I G I T V F Y F S L W P D D A N Q V M ORFD V F K K K P L S C M F G L L P V I Y I L S C M P K N E N D V F GRB2 I F H L K N K N N A W L L T A M L A A T F F I T M Y P K D L N D V F YNDE A F K K Q T Q S G N Y L T P V I V F I T I I I A F I P E S I S D T L GBME L F K I K S P S R L A Y P L G I I V L F L S I T I A S N F Q E H GRKB L F K E K N P S Q L A Y P M G L G I L I L S I T I A T N F S E H L GERI I F R V K Q R G V L I A F M F I T L I V S Q L L T N R H D I N N F YFKT L H S K I D P K A M Y T P T G M F S V V C G F W F F L N T N Q L I GERX V T K Q K A R N Q I I S I L L I C M V I I C I L P I S D A S F Y GRA2 M Y S D Y L G Y I F V S L F L L P F I L F F I V A L K R R ORFC M Y S D Y L G Y I F V S L F L L A V H S L F H V A L K R R ORFD I L G D T V S H I A L Y I F G A L P I L L L V I S K W R K GRB2 Y Y G T L L G Y A F L I V I T I P F F V W F L S W I Q K K YNDE T W G D W I G Y F G F S V L I L S L L V G Y V T V G W R K K R R GBME L E G L H V A R L L L H M P L F A A V P I L L L L I A F F K N R K GRKB N E G L N V V P L Y I H L P F Q L L F P L F L F I V A V W K K K R R E GERI I S N A S K G S F W L I Y V Y I P I L F I I V W V K R K YFKT D F N R I K P I I N V V I S L L L P L L I Y L I I K S K A L F GERX W F L S H V F L P I S A I G L F L F S M L L L V F V W V S GRA2 I T T K ORFC I T T K ORFD R G E K GRB2 I G R G Q L Q YNDE E L G D R H G E S T K A GBME R Q Q R Q GRKB K S K G E E A K K GERI W K K S Q E N YFKT G A K A K H GERX K N K K R A -- Results -- Length of sequences with gaps = 397 Average sequence length: 366 Percent identity (based on aligned regions): GRA2 ORFC ORFD GRB2 YNDE GBME GRKB GERI YFKT GERX GRA2 0.00 97.80 38.44 32.22 24.03 24.08 22.38 21.35 21.68 17.92 ORFC 97.80 0.00 37.71 31.20 24.10 23.51 21.53 20.00 21.45 18.26 ORFD 38.44 37.71 0.00 33.52 25.70 27.35 23.93 20.68 18.84 19.53 GRB2 32.22 31.20 33.52 0.00 24.73 20.40 20.56 19.55 17.92 19.20 YNDE 24.03 24.10 25.70 24.73 0.00 17.13 19.89 17.09 15.54 14.94 GBME 24.08 23.51 27.35 20.40 17.13 0.00 51.37 19.48 22.25 19.25 GRKB 22.38 21.53 23.93 20.56 19.89 51.37 0.00 18.75 24.00 15.47 GERI 21.35 20.00 20.68 19.55 17.09 19.48 18.75 0.00 16.47 13.91 YFKT 21.68 21.45 18.84 17.92 15.54 22.25 24.00 16.47 0.00 15.43 GERX 17.92 18.26 19.53 19.20 14.94 19.25 15.47 13.91 15.43 0.00 Distance scores for phylogenetic tree: GRA2 ORFC ORFD GRB2 YNDE GERI GBME GRKB YFKT GERX GRA2 0.00 3.04 78.48 89.52 118.32 132.62 135.01 140.44 150.39 153.63 ORFC 3.04 0.00 81.84 94.63 120.49 138.48 137.82 144.70 154.03 154.04 ORFD 78.48 81.84 0.00 91.42 113.43 134.19 118.91 133.43 152.70 146.50 GRB2 89.52 94.63 91.42 0.00 123.63 138.23 149.57 149.88 167.00 154.57 YNDE 118.32 120.49 113.43 123.63 0.00 156.44 165.23 159.44 171.65 160.76 GERI 132.62 138.48 134.19 138.23 156.44 0.00 146.56 153.42 166.82 173.12 GBME 135.01 137.82 118.91 149.57 165.23 146.56 0.00 56.44 146.23 147.48 GRKB 140.44 144.70 133.43 149.88 159.44 153.42 56.44 0.00 138.16 160.31 YFKT 150.39 154.03 152.70 167.00 171.65 166.82 146.23 138.16 0.00 176.80 GERX 153.63 154.04 146.50 154.57 160.76 173.12 147.48 160.31 176.80 0.00 Branching order: (GRA2,ORFC)ORFD)GRB2)YNDE)GERI)GBME)GRKB)YFKT)GERX) Clusters: (GBME,GRKB)YFKT) A)GRA2 GRA2BSU.TXT B)ORFC ORFCBSU.TXT C)ORFD ORFDBSU.TXT D)GRB2 GRB2BSU.TXT E)YNDE YNDEBHA.TXT F)GERI GERIBBSU.TXT G)GBME GERABBME.TXT H)GRKB GRKBBSU.TXT I)YFKT YFKTBSU.TXT J)GERX GERXBBAN.TXT Branch lengths: (r1,r2,r3,r4,r5) (r6,r7,........) -0.21 3.25 41.52 37.12 0.15 50.64 6.71 68.68 11.03 74.99 6.04 26.16 30.28 34.43 79.55 8.85 84.40 % s.d. (observed vs calculated) = 2.91