VIDEO

8. Translocation of the PH domain from Akt/PKB to the plasma membrane in response to uniform stimulation

Analysis of the translocation of the PH domain from the serine/threonine kinase Akt/PKB fused to GFP to the plasma membrane in response to uniformly stimulating the cells with the chemoattractant cAMP. Images were taken every 2.6 seconds. Maximal membrane localization occurs approximately 6 seconds after stimulation. The protein is rapidly lost from the membrane and is no longer detected on the membrane by 12 seconds. Akt/PKB is also activated in response to chemoattractant stimulation. Maximal enzyme activity is approximately 10-15 seconds after stimulation. R. Meili, Firtel lab.