IDENTIFICATION OF NEURONS AND NETWORKS INVOLVED IN BEHAVIORAL CHOICE
USING FRET-BASED VOLTAGE SENSITIVE DYES IN THE LEECH CNS
K.L. Briggman1*; H. Abarbanel3,4; W.-B. Kristan2  
1. Computational Neurobiol, UC - San Diego, La Jolla, CA, USA 
2. Biology, UC - San Diego, La Jolla, CA, USA 
3. Physics, UC - San Diego, La Jolla, CA, USA 
4. Marine Physical Laboratory, Scripps Institution of Oceanography,
   La Jolla, CA, USA 
 
A FRET-based voltage sensitive dye was used to record from approximately
100 identifiable neurons simultaneously in the leech segmental ganglion.
We infer the behavioral state of the leech based on this population
recording. A sensory stimulus was delivered, evoking either crawling or
swimming in an isolated nerve cord. We imaged for several seconds pre- 
and post-stimulus to determine the behavioral state of the animal. We 
identified cells involved in the behavioral choice between these two
behaviors. Cell identification was performed using standard 
electrophysiological techniques once a neuron of interest was optically
identified. This high-throughput screening of behaviorally relevant cells
greatly aids in the discovery of new neurons involved in behavioral choice
networks.

Supported by: NIH MH43396 and NS35336

 
 
Citation:
K.L. Briggman, H. Abarbanel, W.-B. Kristan. IDENTIFICATION OF NEURONS AND
NETWORKS INVOLVED IN BEHAVIORAL CHOICE USING FRET-BASED VOLTAGE SENSITIVE
DYES IN THE LEECH CNS Program No. 403.9. 2002 Abstract Viewer/Itinerary
Planner. Washington, DC: Society for Neuroscience, 2002. Online.