Protocols

Molecular biology

polymerase chain reaction (PCR)		rapid transformant screening
in-gel ligations		rapid plasmid miniprep
electrocompetent cells		silica-based miniprep
electroporation		plasmid DNA prep (clean)
cosmid prep		RNA Synthesis
gel purification of DNA fragments		ResGen Oligos
clean-up of DNA from PCR/agarose gel/restriction digests		cDNA library amplification

Protein methods

SDS-PAGE		Immunoprecipitations
Electroblotting of Proteins
Western Blotting		MBP Fusion Protein Purification
Affinity Purification of Peptide Antibodies		GST Fusion Protein Purification
Bt-toxin lysate preparation		Affinity Purification of Antibodies Using GST Fusion Proteins

 

C. elegans techniques

Freezing worms
Lineaging embryos		Nematode genomic DNA isolation
Freeze-fracture		Single Worm PCR
Basic immunofluorescence		RNA-injection
Fixation & Staining Parameters		Synchronizing Worm Populations
POD-1 stainings		EMS Mutagenesis
Making transgenic nematodes		Genetic screen for osmosensitive mutants
RNA-isolation (TRIZOL method)		snip-SNP mapping
PAR stainings (as per K. Kemphues) PAR-1 PAR-2 PAR-3		C. elegans extract preparation

 

 

M. incognita and plant techniques

M. incognita Protocols

embryo buffers		plates for worms
protease inhibitors

Bacillus thuringiensis techniques

Staining, extracting, and sporulation