M W F; 10:10 - 11:00 pm

 Molecular Biology

 Douglas W. Smith

York 2722

 BIMM 100

 5254 Muir Biology Building

Fall, 2000  

 x42620; dsmith@ucsd.edu

 

Lectures

 

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The following links are to Lecture Notes for BIMM 100, Fall, 2000.

The Lecture Notes below on the Web are those modified throughout the Fall 2000 course, to reflect the shift in emphasis from a Genes oriented course to a Genome oriented course

NOTE: The designation of which Topics are covered in a given Lecture is only approximate. Due to a variety of circumstances, a particular Topic may be covered in a Lecture different from that shown below, or in some cases may not explicitly be covered in Lecture.

You are still responsible for material in the Lecture Notes even if not covered explicitly in lecture.

When Lecture Notes for Fall, 2000, are made available, there will be a link below to those Lecture Notes.

 

Topics

 Lecture

 Date

 Topic

 1

F 22 Sep

 Mol Biol; Genomes
 Mol Biol; Genetic Material; Nucleic Acid Structure; Genes

 2

M 25 Sep

  Genome Mapping: Physical - Low Resolution
 R. enzymes; Modification; Gels; Hybridization; Restriction Maps; DNA Chips

3

W 27 Sep

  Genome Mapping: Cloning - High Resolution
 Cloning; Vectors; Reverse Genetics; Libraries; ESTs; Rad Hybrids; STSs

4

F 29 Sep

  Genome Mapping: Genetic
 Physical / Genetic Maps; DNA Markers (RFLPs, SSLPs, SNPs); PCR; Pedigree

 5

 M 2 Oct

  Genome Sequencing
 Chemical / Dideoxy; Automated; Genome Assembly; Shotgun; Clones; Markers

 6

 W 4 Oct

  Functional Genomics: Basics
 Genome Element (Genes / Sites) Location and Function; Computational / Expt

 7

 F 6 Oct

  Genome Anatomies: Prokaryotic
 DNA Supercoils, Topos; Bacterial Nucleoid; Packing Ratio; Mito DNA

 8

 M 9 Oct

  Genome Anatomies: Eukaryotic
 Chromatin; Nucleosomes; 30 nm Fiber; MARs, SARs

9

W 11 Oct

  Genome Anatomies: Mobile Elements
 Prok IS, Tns; Transposition; Euk Tns; Retrotransposons; LINEs, SINEs

10

F 13 Oct

  Genome Function: DNA-binding Proteins
This material will be incorporated within other lectures

11

M 16 Oct

  Genome Duplication: DNA replication - Basics
  Principles, Meselson-Stahl, SemiDiscontinuous Synthesis; Polymerases

12

W 18 Oct

  Genome Duplication: DNA replication - Initiation and Elongation
 Elongation Process, Proteins, Euk vs Prok, Replication Origins

13

F 20 Oct

  Genome Duplication: DNA replication - Termination
 Prokaryotic Termination; Telomeres; Telomerase

14

M 23 Oct

  Genome Evolution: Mutations
This material is review material from Genetics, with some in DNA Repair lec

15

W 25 Oct

  Genome Evolution: DNA Repair
Mutations; Direct Repair; Nucleotide Excision; Base Excision; Mismatch; SOS

16

F 27 Oct

  Genome Evolution: Homologous Recombination
 Breakage-Reunion; Meselson-Radding; Enzymes; Gene Conversion

17

M 30 Oct

  Genome Function: Prokaryotic RNA Transcription
RNA pol; Sigmasss; Promoters; DNA footprinting; Elongation; Termination

18

W 1 Nov

  Genome Function: Prokaryotic Transcription Regulation
Operon Theory: Catabolite (Lac), Anabolic (Trp); Genetics; CAP; Attenuation

 19

 F 3 Nov

  Genome Function: Eukaryotic RNA Transcription
 Basics; RNA pols I, II, III; Methodologies

20

 M 6 Nov

  Genome Function: Eukaryotic Transcription Regulation
Sites, Proximal, Enhancers; Response Elements; Transcrip Factors; Two Hybrid

 21

 W 8 Nov

  Genome Function: RNA Processing
 hnRNA; G Cap; poly(A); Splicing; Editing

22

M 13 Nov

  Genome Function: RNA Splicing
GU-AG; Spliceosomes; snRNPs; RNaseP; Ribozymes; L-19 RNA

23

W 15 Nov

  Genome Function: Total Expression
 This material will be incorporated within other lectures

 24

F 17 Nov

  Proteome Synthesis: Genetic Codes
Basics; Crick T4 expt; Codons IDs; NonUniversality

25

M 20 Nov

  Proteome Synthesis: tRNA, Ribosomes
 Translation Basics; tRNA: Adaptor, Structure, Function, Wobble; Ribosomes

26

W 22 Nov

  Proteome Synthesis: Translation and Processing
 Initiation, Prok vs Euk; Elongation; Termination; Nonsense Suppressors

27

M 27 Nov

  PostGenome Molecular Biology and Genome Evolution
 Lander 10 Goals; RNA World; Genome Duplication; Gene Duplication

28

W 29 Nov

  Genome Evolution: Phylogenetics
 Multiple Sequence Alignments; Trees; Bootstrapping; Human Prehistory

 29

 F 1 Dec

  Bioinformatics: Databases and Software Tools
Bioinformatics, Software, Sequence Databases, Organism Databases

 


| BIMM100 | Syllabus | Sections / Off Hrs | Grading Policy | DNASYSTEM |
| Lectures | Journal Articles | Study Qs | Lab Techniques | Exams |

 

If you have problems or comments, send email to Doug Smith