Localization of a PH domain-containing
protein (phdA) to the plasma membrane in response to changes in the direction
of the cAMP source: Small version
Time-lapse video movie of a GFP fusion
of a novel PH domain-containing protein (phdA), which is required for proper
chemotaxis, to the plasma membrane in response to directional changes in
the cAMP source. The position of the micropipette is indicated by a star.
Note that as the position of the micropipette is moved, the GFP fusion
localizes to a position in the plasma membrane closest to the cAMP source
and is lost from the membrane at its previous site. In some cases, a new
pseudopod extends shortly after membrane localization of the protein and
retracts as the micropipette containing cAMP is moved. S. Funamoto, Firtel
lab.