Localization of F-actin in ARK1
null cells chemotaxing towards cAMP visualized using ABP-GFP: Large version
Time-lapse video microscopy of ARK1 null
cells expressing a GFP fusion of the F-actin binding domain from ABP120
(a gift from David Knecht, Univ. of Conn.). ARK1, an ankyrin-repeat containing
kinase is required for proper chemotaxis. The GFP fusion of the actin binding
domain from ABP120 is used to visualize F-actin in vivo. The movie
shows the localization of the F-actin in these cells. Note the very broad
leading edge and multiple pseudopodia in many of the ARK1 null cells that
are not usually seen in wild-type cells. B. Sun, Firtel lab.