Translocation of the PH domain
from Akt/PKB to the plasma membrane in response to uniform stimulation:
Large version
Analysis of the translocation of the PH
domain from the serine/threonine kinase Akt/PKB fused to GFP to the plasma
membrane in response to uniformly stimulating the cells with the chemoattractant
cAMP. Images were taken every 2.6 seconds. Maximal membrane localization
occurs approximately 6 seconds after stimulation. The protein is rapidly
lost from the membrane and is no longer detected on the membrane by 12
seconds. Akt/PKB is also activated in response to chemoattractant stimulation.
Maximal enzyme activity is approximately 10-15 seconds after stimulation.
R. Meili, Firtel lab.