Multiplex Fluorescent mRNA In Situ Hybridization

9/26/03: This site is under construction, but is nearly complete. It presents information on how to do multiplex fluorescent mRNA in situ hybridization on Drosophila embryos. Questions, suggestions, or comments, please e-mail me at davek@biomail.ucsd.edu. The following links provide protocols, images, and general information on this project:

Introduction
Embryo Gallery
Protocol Overview
Embryo Fixation
Embryo Treatment Before Hybridization
Hybridization and Making RNA Probes
Post-Hybridization Fluorescent Detection
Presentation of Embryos for Fluorescence Microscopy
Simultaneous Detection of mRNA and Protein
Alternative Methods for Fluorescent Detection
Nuclear Dots and Virtual Colors
Conclusions and Future Problems
Reagents and Recipes
Links and References
Questions
Coming soon: Projects

Prepared by Dave Kosman (Ethan Bier and Bill McGinnis labs, UC San Diego)
Protocols developed in collaboration with Mieko Mizutani and Greg Cox
Project supported by NSF IBN0120728